Overall, this is an excellent review of the state of the art of MEAs for 2D in vitro culture systems. The specific suggestions below are relatively minor. The author, Dr. Gross, is the foremost pioneer in this area and exceptionally qualified to provide this review. One substantitive suggestion is that the author is encouraged to include reference(s) to recent and promising work by Claverol-Tinture (e.g., Integrating multi-unit electrophysiology and plastic culture dishes for network neuroscience (LAB ON A CHIP, 9(4): 619-619, 2009).
1. Please edit "Dissociate embryonic tissue forms" to "Dissociated embryonic tissue forms"
2. Re: The adhered ”monolayer” is actually a shallow 3-dimensional volume with neurons residing on top of a glial carpet and neurites both on top and below the carpet. Please provide thickness of the insulating layer and any dimensions concerning the typical 3D volumes.
3. Re: "Complete control over this stratification and the glial capping has not yet been achieved, but would yield very large signals (of the order of 1 mV) and a high electrode yield." -- suggest softening this statement and/or citing papers that support the claim.
An excellent article on passive planar MEA technology with thorough review of results by the author’s group.
Fig. 4: The author state: “Over 70% of the recordings are obtained from axons.” This statement suggests that the source of an MEA signal is usually known. While the spike shape and size usually suggests axon or dendrite, the complex geometry of the neuron and its relationship to spike temporal profile makes it difficult to infere the cell compartment which originates the signal. This statement should be taken as indicating the general view in the field that most large MEA signals are associated with axon hillocks or other active axonal segments.
Minor typographic corrections:
"Indium-tin oxide" and "inditum-tin oxide", capital "I" and low-case "i" are both used. For consistency one should be used.
Please consider rephrasing “systematic fabrications”, e.g. “large-scale production”, “systematic production”, “systematic fabrication”, etc.
The following text includes control tags that likely should be removed : <ref name="Crain"> Crain SM (1976) Neurophysiologic Studies in Tissue Culture. Raven Press, NY.</ref>